Protein_Domain

Part:BBa_M36515:Design

Designed by: Kevin Bui, Jordan Shapiro, Kevin Madrigal   Group: Stanford BIOE44 - S11   (2012-12-06)


Silaffin 1 Peptide Encoding Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 360
    Illegal SapI.rc site found at 426


Design Notes

The silaffin 1 peptide amino acid sequence found in marine diatoms can be found online at http://www.uniprot.org/uniprot/Q9SE35. Our project entailed expressing silaffin 1 in E. coli, thus we derived the DNA sequence from the online amino acid sequence and codon optimized the sequence for E. coli. We also optimized to eliminate repeats and hidden stop codons.


Source

http://www.uniprot.org/uniprot/Q9SE35 This is the source of the amino acid sequence of silaffin 1 in marine diatoms. We reverse engineered this sequence to create our gene for E. coli

References